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stx4a  (Santa Cruz Biotechnology)


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    Structured Review

    Santa Cruz Biotechnology stx4a
    Stx4a, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 92/100, based on 27 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/stx4a/Syntaxin+4+Antibody/pm39358505-884-46-47
    Average 92 stars, based on 27 article reviews
    stx4a - by Bioz Stars, 2026-09
    92/100 stars

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    Related Articles

    Blocking Assay:

    Article Title: CRISPR–Cas9 screens reveal regulators of ageing in neural stem cells
    Article Snippet: Slides were treated with 50–70 μl blocking solution (5% normal donkey serum (NDS, ImmunoReagents, SP-072-VX10), 1% BSA (Sigma-Aldrich, A1595-50ML), 8.5 ml PBS) in a humidified chamber at room temperature for 30 min. .. Blocking solution was replaced with antibody solution consisting of blocking solution with antibodies as follows: mCherry (Invitrogen, M11217, clone 16D7) 1:500, GFAP (Abcam, 53554) 1:500, GFP (Abcam, 13970) 1:500, GLUT4 (for in vivo staining R&D Systems, MAB1262, clone 1F8) 1:500, Ki67 (Invitrogen, 14-5698-082, clone SolA15) 1:500, STX4A (Santa Cruz Biotechnology, sc-101301, clone QQ-17) 1:500, GFP (Abcam, 13970) 1:500, mouse IgG (Santa Cruz SC-3877, lot: L1916) 1:500, NeuN (Millipore, MAB377 clone A60) 1:500, S100a6 (Abcam, ab181975, clone EPR13084-69) 1:500, Tuj1 (BioLegend, 802001) 1:500, Olig2 (R&D Systems, AF2418) 1:100, Sox10 (Abcam, Ab180862, clone EPR4007-104) 1:100, calretinin (Abcam, Ab244299) 1:500, Dcx (Cell Signaling Technology, 4604) 1:500. .. We tested two mCherry antibodies (Abcam, ab213511 clone EPR20579; Invitrogen, M11217 clone 16D7) and we found that Invitrogen, M11217 was better for immunostaining for brain sections.

    Article Title: CRISPR-Cas9 screens reveal regulators of ageing in neural stem cells.
    Article Snippet: Slides were treated with 50–70 μl blocking solution (5% normal donkey serum (NDS, ImmunoReagents, SP-072-VX10), 1% BSA (Sigma-Aldrich, A1595-50ML), 8.5 ml PBS) in a humidified chamber at room temperature for 30 min. .. Blocking solution was replaced with antibody solution consisting of blocking solution with antibodies as follows: mCherry (Invitrogen, M11217, clone 16D7) 1:500, GFAP (Abcam, 53554) 1:500, GFP (Abcam, 13970) 1:500, GLUT4 (for in vivo staining R&D Systems, MAB1262, clone 1F8) 1:500, Ki67 (Invitrogen, 14-5698-082, clone SolA15) 1:500, STX4A (Santa Cruz Biotechnology, sc-101301, clone QQ-17) 1:500, GFP (Abcam, 13970) 1:500, mouse IgG (Santa Cruz SC-3877, lot: L1916) 1:500, NeuN (Millipore, MAB377 clone A60) 1:500, S100a6 (Abcam, ab181975, clone EPR1308469) 1:500, Tuj1 (BioLegend, 802001) 1:500, Olig2 (R&D Systems, AF2418) 1:100, Sox10 (Abcam, Ab180862, clone EPR4007-104) 1:100, calretinin (Abcam, Ab244299) 1:500, Dcx (Cell Signaling Technology, 4604) 1:500. .. We tested two mCherry antibodies (Abcam, ab213511 clone EPR20579; Invitrogen, M11217 clone 16D7) and we found that Invitrogen, M11217 was better for immunostaining for brain sections.

    Article Title: In vitro and in vivo CRISPR-Cas9 screens reveal drivers of aging in neural stem cells of the brain
    Article Snippet: Slides were treated with 50-70 μL blocking solution (5% normal donkey serum [NDS, ImmunoReagents, SP-072-V×10], 1% Bovine Serum Albumin (BSA, Sigma-Aldrich, A1595-50ML), 8.5 mL PBS) in a humidified chamber at room temperature for 30 minutes. .. Blocking solution was replaced with antibody solution consisting of blocking solution with antibodies as follows: mCherry (Invitrogen, M11217) 1:500, GFAP (Abcam, 53554) 1:500, GFP (Abcam, 13970) 1:500, GLUT4 (for in vivo staining R&D, MAB1262) 1:500, Ki67 (Invitrogen, 14-5698-082) 1:500, STX4A (Santa Cruz Biotechnology, sc-101301) 1:500, GFP (Abcam, 13970) 1:500, mouse IgG (Santa Cruz SC-3877, Lot: L1916) 1:500. ..

    In Vivo:

    Article Title: CRISPR–Cas9 screens reveal regulators of ageing in neural stem cells
    Article Snippet: Slides were treated with 50–70 μl blocking solution (5% normal donkey serum (NDS, ImmunoReagents, SP-072-VX10), 1% BSA (Sigma-Aldrich, A1595-50ML), 8.5 ml PBS) in a humidified chamber at room temperature for 30 min. .. Blocking solution was replaced with antibody solution consisting of blocking solution with antibodies as follows: mCherry (Invitrogen, M11217, clone 16D7) 1:500, GFAP (Abcam, 53554) 1:500, GFP (Abcam, 13970) 1:500, GLUT4 (for in vivo staining R&D Systems, MAB1262, clone 1F8) 1:500, Ki67 (Invitrogen, 14-5698-082, clone SolA15) 1:500, STX4A (Santa Cruz Biotechnology, sc-101301, clone QQ-17) 1:500, GFP (Abcam, 13970) 1:500, mouse IgG (Santa Cruz SC-3877, lot: L1916) 1:500, NeuN (Millipore, MAB377 clone A60) 1:500, S100a6 (Abcam, ab181975, clone EPR13084-69) 1:500, Tuj1 (BioLegend, 802001) 1:500, Olig2 (R&D Systems, AF2418) 1:100, Sox10 (Abcam, Ab180862, clone EPR4007-104) 1:100, calretinin (Abcam, Ab244299) 1:500, Dcx (Cell Signaling Technology, 4604) 1:500. .. We tested two mCherry antibodies (Abcam, ab213511 clone EPR20579; Invitrogen, M11217 clone 16D7) and we found that Invitrogen, M11217 was better for immunostaining for brain sections.

    Article Title: CRISPR-Cas9 screens reveal regulators of ageing in neural stem cells.
    Article Snippet: Slides were treated with 50–70 μl blocking solution (5% normal donkey serum (NDS, ImmunoReagents, SP-072-VX10), 1% BSA (Sigma-Aldrich, A1595-50ML), 8.5 ml PBS) in a humidified chamber at room temperature for 30 min. .. Blocking solution was replaced with antibody solution consisting of blocking solution with antibodies as follows: mCherry (Invitrogen, M11217, clone 16D7) 1:500, GFAP (Abcam, 53554) 1:500, GFP (Abcam, 13970) 1:500, GLUT4 (for in vivo staining R&D Systems, MAB1262, clone 1F8) 1:500, Ki67 (Invitrogen, 14-5698-082, clone SolA15) 1:500, STX4A (Santa Cruz Biotechnology, sc-101301, clone QQ-17) 1:500, GFP (Abcam, 13970) 1:500, mouse IgG (Santa Cruz SC-3877, lot: L1916) 1:500, NeuN (Millipore, MAB377 clone A60) 1:500, S100a6 (Abcam, ab181975, clone EPR1308469) 1:500, Tuj1 (BioLegend, 802001) 1:500, Olig2 (R&D Systems, AF2418) 1:100, Sox10 (Abcam, Ab180862, clone EPR4007-104) 1:100, calretinin (Abcam, Ab244299) 1:500, Dcx (Cell Signaling Technology, 4604) 1:500. .. We tested two mCherry antibodies (Abcam, ab213511 clone EPR20579; Invitrogen, M11217 clone 16D7) and we found that Invitrogen, M11217 was better for immunostaining for brain sections.

    Article Title: In vitro and in vivo CRISPR-Cas9 screens reveal drivers of aging in neural stem cells of the brain
    Article Snippet: Slides were treated with 50-70 μL blocking solution (5% normal donkey serum [NDS, ImmunoReagents, SP-072-V×10], 1% Bovine Serum Albumin (BSA, Sigma-Aldrich, A1595-50ML), 8.5 mL PBS) in a humidified chamber at room temperature for 30 minutes. .. Blocking solution was replaced with antibody solution consisting of blocking solution with antibodies as follows: mCherry (Invitrogen, M11217) 1:500, GFAP (Abcam, 53554) 1:500, GFP (Abcam, 13970) 1:500, GLUT4 (for in vivo staining R&D, MAB1262) 1:500, Ki67 (Invitrogen, 14-5698-082) 1:500, STX4A (Santa Cruz Biotechnology, sc-101301) 1:500, GFP (Abcam, 13970) 1:500, mouse IgG (Santa Cruz SC-3877, Lot: L1916) 1:500. ..

    Article Title: CRISPR-Cas9 screens reveal regulators of ageing in neural stem cells.
    Article Snippet: Alexa 488/594/647 conjugated antibodies, (Fisher Scientific, A21206, A21209, A31571) 1:500. .. Immunofluorescence straining in brain sections in vivo: mCherry (Invitrogen, M11217 clone 16D7) 1:500, GFAP (Abcam, 53554) 1:500, GFP (Abcam, 13970) 1:500, GLUT4 (R&D Systems, MAB1262 clone 1F8) 1:500, Ki67 (Invitrogen, 14-5698-082 clone SolA15) 1:500, STX4A (Santa Cruz Biotechnology, sc-101301 clone QQ-17) 1:500, GFP (Abcam, 13970) 1:500, mouse IgG (Santa Cruz SC-3877, Lot: L1916) 1:500. .. Alexa 488/594/647 conjugated antibodies, (Fisher Scientific, A21202, A21206, A21209, A21447, A31571, A31573) 1:500, NeuN (Millipore, MAB377, clone A60) 1:500, S100a6 (Abcam, ab181975 clone EPR13084-69) 1:500, Tuj1 (Biolegend, 802001) 1:500, Olig2 (R&D Systems, AF2418) 1:100, Sox10 (Abcam, Ab180862 clone EPR4007-104) 1:100, Calretinin (Abcam, Ab244299) 1:500, Dcx (Cell Signalling Technology, 4604) 1:500.

    Staining:

    Article Title: CRISPR–Cas9 screens reveal regulators of ageing in neural stem cells
    Article Snippet: Slides were treated with 50–70 μl blocking solution (5% normal donkey serum (NDS, ImmunoReagents, SP-072-VX10), 1% BSA (Sigma-Aldrich, A1595-50ML), 8.5 ml PBS) in a humidified chamber at room temperature for 30 min. .. Blocking solution was replaced with antibody solution consisting of blocking solution with antibodies as follows: mCherry (Invitrogen, M11217, clone 16D7) 1:500, GFAP (Abcam, 53554) 1:500, GFP (Abcam, 13970) 1:500, GLUT4 (for in vivo staining R&D Systems, MAB1262, clone 1F8) 1:500, Ki67 (Invitrogen, 14-5698-082, clone SolA15) 1:500, STX4A (Santa Cruz Biotechnology, sc-101301, clone QQ-17) 1:500, GFP (Abcam, 13970) 1:500, mouse IgG (Santa Cruz SC-3877, lot: L1916) 1:500, NeuN (Millipore, MAB377 clone A60) 1:500, S100a6 (Abcam, ab181975, clone EPR13084-69) 1:500, Tuj1 (BioLegend, 802001) 1:500, Olig2 (R&D Systems, AF2418) 1:100, Sox10 (Abcam, Ab180862, clone EPR4007-104) 1:100, calretinin (Abcam, Ab244299) 1:500, Dcx (Cell Signaling Technology, 4604) 1:500. .. We tested two mCherry antibodies (Abcam, ab213511 clone EPR20579; Invitrogen, M11217 clone 16D7) and we found that Invitrogen, M11217 was better for immunostaining for brain sections.

    Article Title: CRISPR-Cas9 screens reveal regulators of ageing in neural stem cells.
    Article Snippet: Slides were treated with 50–70 μl blocking solution (5% normal donkey serum (NDS, ImmunoReagents, SP-072-VX10), 1% BSA (Sigma-Aldrich, A1595-50ML), 8.5 ml PBS) in a humidified chamber at room temperature for 30 min. .. Blocking solution was replaced with antibody solution consisting of blocking solution with antibodies as follows: mCherry (Invitrogen, M11217, clone 16D7) 1:500, GFAP (Abcam, 53554) 1:500, GFP (Abcam, 13970) 1:500, GLUT4 (for in vivo staining R&D Systems, MAB1262, clone 1F8) 1:500, Ki67 (Invitrogen, 14-5698-082, clone SolA15) 1:500, STX4A (Santa Cruz Biotechnology, sc-101301, clone QQ-17) 1:500, GFP (Abcam, 13970) 1:500, mouse IgG (Santa Cruz SC-3877, lot: L1916) 1:500, NeuN (Millipore, MAB377 clone A60) 1:500, S100a6 (Abcam, ab181975, clone EPR1308469) 1:500, Tuj1 (BioLegend, 802001) 1:500, Olig2 (R&D Systems, AF2418) 1:100, Sox10 (Abcam, Ab180862, clone EPR4007-104) 1:100, calretinin (Abcam, Ab244299) 1:500, Dcx (Cell Signaling Technology, 4604) 1:500. .. We tested two mCherry antibodies (Abcam, ab213511 clone EPR20579; Invitrogen, M11217 clone 16D7) and we found that Invitrogen, M11217 was better for immunostaining for brain sections.

    Article Title: In vitro and in vivo CRISPR-Cas9 screens reveal drivers of aging in neural stem cells of the brain
    Article Snippet: Slides were treated with 50-70 μL blocking solution (5% normal donkey serum [NDS, ImmunoReagents, SP-072-V×10], 1% Bovine Serum Albumin (BSA, Sigma-Aldrich, A1595-50ML), 8.5 mL PBS) in a humidified chamber at room temperature for 30 minutes. .. Blocking solution was replaced with antibody solution consisting of blocking solution with antibodies as follows: mCherry (Invitrogen, M11217) 1:500, GFAP (Abcam, 53554) 1:500, GFP (Abcam, 13970) 1:500, GLUT4 (for in vivo staining R&D, MAB1262) 1:500, Ki67 (Invitrogen, 14-5698-082) 1:500, STX4A (Santa Cruz Biotechnology, sc-101301) 1:500, GFP (Abcam, 13970) 1:500, mouse IgG (Santa Cruz SC-3877, Lot: L1916) 1:500. ..

    Chromatin Immunoprecipitation:

    Article Title: CRISPR-Cas9 screens reveal regulators of ageing in neural stem cells.
    Article Snippet: .. 3 nature portfolio | reporting sum m ary April 2023 Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Clinical data Dual use research of concern Plants Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used Immunofluorescence: In vitro: GLUT4 (Abcam, 33780) 1:500, Ki67 (Invitrogen, 14-5698-082 clone SolA15) 1:500, STX4A (Santa Cruz Biotechnology, SC101301 clone QQ-17) 1:500. .. Alexa 488/594/647 conjugated antibodies, (Fisher Scientific, A21206, A21209, A31571) 1:500.

    Flow Cytometry:

    Article Title: CRISPR-Cas9 screens reveal regulators of ageing in neural stem cells.
    Article Snippet: .. 3 nature portfolio | reporting sum m ary April 2023 Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Clinical data Dual use research of concern Plants Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used Immunofluorescence: In vitro: GLUT4 (Abcam, 33780) 1:500, Ki67 (Invitrogen, 14-5698-082 clone SolA15) 1:500, STX4A (Santa Cruz Biotechnology, SC101301 clone QQ-17) 1:500. .. Alexa 488/594/647 conjugated antibodies, (Fisher Scientific, A21206, A21209, A31571) 1:500.

    Magnetic Resonance Imaging:

    Article Title: CRISPR-Cas9 screens reveal regulators of ageing in neural stem cells.
    Article Snippet: .. 3 nature portfolio | reporting sum m ary April 2023 Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Clinical data Dual use research of concern Plants Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used Immunofluorescence: In vitro: GLUT4 (Abcam, 33780) 1:500, Ki67 (Invitrogen, 14-5698-082 clone SolA15) 1:500, STX4A (Santa Cruz Biotechnology, SC101301 clone QQ-17) 1:500. .. Alexa 488/594/647 conjugated antibodies, (Fisher Scientific, A21206, A21209, A31571) 1:500.

    Immunofluorescence:

    Article Title: CRISPR-Cas9 screens reveal regulators of ageing in neural stem cells.
    Article Snippet: .. 3 nature portfolio | reporting sum m ary April 2023 Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Clinical data Dual use research of concern Plants Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used Immunofluorescence: In vitro: GLUT4 (Abcam, 33780) 1:500, Ki67 (Invitrogen, 14-5698-082 clone SolA15) 1:500, STX4A (Santa Cruz Biotechnology, SC101301 clone QQ-17) 1:500. .. Alexa 488/594/647 conjugated antibodies, (Fisher Scientific, A21206, A21209, A31571) 1:500.

    Article Title: CRISPR-Cas9 screens reveal regulators of ageing in neural stem cells.
    Article Snippet: Alexa 488/594/647 conjugated antibodies, (Fisher Scientific, A21206, A21209, A31571) 1:500. .. Immunofluorescence straining in brain sections in vivo: mCherry (Invitrogen, M11217 clone 16D7) 1:500, GFAP (Abcam, 53554) 1:500, GFP (Abcam, 13970) 1:500, GLUT4 (R&D Systems, MAB1262 clone 1F8) 1:500, Ki67 (Invitrogen, 14-5698-082 clone SolA15) 1:500, STX4A (Santa Cruz Biotechnology, sc-101301 clone QQ-17) 1:500, GFP (Abcam, 13970) 1:500, mouse IgG (Santa Cruz SC-3877, Lot: L1916) 1:500. .. Alexa 488/594/647 conjugated antibodies, (Fisher Scientific, A21202, A21206, A21209, A21447, A31571, A31573) 1:500, NeuN (Millipore, MAB377, clone A60) 1:500, S100a6 (Abcam, ab181975 clone EPR13084-69) 1:500, Tuj1 (Biolegend, 802001) 1:500, Olig2 (R&D Systems, AF2418) 1:100, Sox10 (Abcam, Ab180862 clone EPR4007-104) 1:100, Calretinin (Abcam, Ab244299) 1:500, Dcx (Cell Signalling Technology, 4604) 1:500.

    In Vitro:

    Article Title: CRISPR-Cas9 screens reveal regulators of ageing in neural stem cells.
    Article Snippet: .. 3 nature portfolio | reporting sum m ary April 2023 Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Clinical data Dual use research of concern Plants Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used Immunofluorescence: In vitro: GLUT4 (Abcam, 33780) 1:500, Ki67 (Invitrogen, 14-5698-082 clone SolA15) 1:500, STX4A (Santa Cruz Biotechnology, SC101301 clone QQ-17) 1:500. .. Alexa 488/594/647 conjugated antibodies, (Fisher Scientific, A21206, A21209, A31571) 1:500.



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